We are looking for an enthusiastic chemist post-doctoral fellow to join our on-going molecular biotechnology project in the field of natural products of high pharmacologic interest. We study cyanobactins, Ribosomally synthesized and Post-translationally modified Peptides (RiPPs), which are produced by pathways involving successive enzymatic steps acting on a peptide precursor. Cyanobactins cannot be produced in large amounts by chemical synthesis. This drawback can be circumvented by rebuilding in-vitro their complete biosynthetic pathway using recombinant enzymes produced individually in E. coli. Following this rationale, our lab has started rebuilding two such biosynthetic pathways for the production of cyanobactins from Microcystis aeruginosa.
The candidate will have a key role in our biotechnology projects, as his/her chemistry skills will be required daily in order to monitor enzymatic activities & characterize intermediate compounds of the biosynthetic pathways. Our ultimate goal will be to purify preparative amounts (i.e. mg) of mature cyanobactins that will further undergo pharmacologic activity assays. Far beyond chemistry bench skills, candidate will be in charge of providing a decisive intellectual contribution within a “three-part task force” combining genomic, molecular biotechnology and chemistry. Candidate should therefore show high motivation in daily challenging situations.