Our lab studies the mechanisms that lead to testicular pathophysiologies such as fertility disorders or testicular germ cell cancers. We are interested in deciphering the impacts of altered metabolism and/or of exposures to environmental molecules on testicular physiology. In order to perform such work, we are using pharmacological approaches combined with specific genetic models such as C. elegans, transgenic mice, or culture cell of tumor cell lines.
To achieve this project, we will use genetically modified mice that are predisposed to TGCT and testicular organotypic culture system. In addition, we will develop single-cell approach to decipher the molecular mechanisms involved in tumor development, aggressiveness, or chemo-resistance. This analysis will be key to study switches of homeostasis and metabolism between normal to tumor cells. Through these models combined with high-throughput approaches (such as RNAseq), candidate will analyze the biology of germ cell tumors (initiation, progression, and invasion) as well as their sensitivity to therapy in order to decipher the roles of targeted signaling pathways. In addition, the candidate will use C. elegans as a powerful genetic model to validate candidates defined in mouse models. This transposition will be useful to develop a new model to study germ cell tumors in regards to the 3R ethical rules.
The validation of these models will allow us to first extend their use in the context of TGCT biology in order to provide mechanistic connections between selected signaling pathways and TGCT etiology. Secondly, this work should provide new insights for providing novel prognostic markers and potential therapeutic targets.
Qualified candidates should be self-driven and highly motivated individuals with an established track record of success, including first-author publications. Experience in cancer biology, developmental biology, reproductive biology, cell, and molecular biology, or related field(s) is desirable. The candidate must have experience in genetically modified mouse models and/or C. elegans biology, cell culture, single-cell and molecular biology techniques (RNAseq, etc.), bioinformatics skills.